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biotium permeabilization buffer (Biotium)

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Biotium biotium permeabilization buffer
Biotium Permeabilization Buffer, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer/Permeabilization+Buffer/pm42092682-394-6-6
Average 94 stars, based on 32 article reviews

biotium permeabilization buffer - by Bioz Stars, 2026-10

94/100 stars

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Article Title: An integrated single cell and spatial transcriptomic map of human white adipose tissue
Article Snippet: After incubating the cell suspensions for 30 minutes at room temperature in the dark, they were washed with permeabilization buffer (#00-8333-56, eBioscience), centrifuged at 400 × g for 5 minutes, and resuspended with an intracellular staining cocktail in 1× permeabilization buffer for 30 minutes at room temperature in the dark. .. Lastly, the cells were washed with permeabilization buffer and fixed with 1% PFA (#22023-20 ml, Biotium) for 15 minutes prior to analysis with a BD Symphony analyzer equipped with 355, 405, 488, 561 and 640 nm lasers and DIVA software (BD Biosciences). .. Fixable live/dead Aqua stain (#L34957, Invitrogen) was included in the surface stain cocktail and used for dead cell exclusion.

Article Title: An integrated single cell and spatial transcriptomic map of human white adipose tissue.
Article Snippet: After incubating the cell suspensions for 30minutes at room temperature in the dark, they were washed with permeabilization buffer (#00-8333-56, eBioscience), centrifuged at 400 × g for 5 minutes, and resuspended with an intracellular staining cocktail in 1× permeabilization buffer for 30minutes at room temperature in the dark. .. Lastly, the cells were washed with permeabilization buffer and fixed with 1% PFA (#22023-20ml, Biotium) for 15minutes prior to analysis with a BD Symphony analyzer equippedwith 355, 405, 488, 561 and640 nm lasers andDIVA software (BD Biosciences). .. Fixable live/dead Aqua stain (#L34957, Invitrogen) was included in the surface stain cocktail and used for dead cell exclusion.

Conjugation Assay:

Article Title: Didemnin B and ternatin-4 differentially inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes
Article Snippet: .. Samples in 25 μl permeabilization buffer were subjected to copper-catalyzed alkyne-azide conjugation to CF405M-azide (Biotium) by addition of 100 μl click reaction mix (50 mM HEPES pH 7.5, 150 mM NaCl, 400 μM TCEP, 250 μM TBTA, 200 μM CuSO 4 , 5 μM azide). ..

Saline:

Article Title: The relationship between acid-sensing ion channel, ASIC2 , and oncogenic β-catenin signaling in ovarian cancer
Article Snippet: .. Cells were then washed in Phosphate Buffered Saline (PBS) X3 and permeabilized with Permeabilization Buffer (Biotium) for 10 min at RT. ..

Article Title: The relationship between acid-sensing ion channel, ASIC2, and oncogenic β-catenin signaling in ovarian cancer.
Article Snippet: .. Cells were then washed in Phosphate Buffered Saline (PBS) X3 and permeabilized with Permeabilization Buffer (Biotium) for 10 min at RT. ..

Staining:

Article Title: Functional SARS-CoV-2 cross-reactive CD4 + T cells established in early childhood decline with age
Article Snippet: .. Stained cells were then washed in permeabilization buffer and fixed with PBS containing 1% paraformaldehyde (Biotium) prior to acquisition. .. Data were acquired using FACSymphony A5 (BD) and analyzed using FlowJo 10.8.1 (FlowJo, LLC).